BACKGROUND

Formalin, a water solution of formaldehyde, is extensively used in laboratories of histopathology, so that each one of them currently employs approximately 5,000 liters of formalin each year. Despite its definite advantages in the preservation of the structural and molecular details, allowing diagnostic precision, the reagent is notoriously toxic and has been recently recognized as carcinogenic. In the European Union, its use has been prohibited starting from August 2017 (see EU Regulations n. 1907/2006 and 1272/2008). At present, however, there is no valid alternative accepted by the scientific community. Therefore, the reagent is still ordinarily used worldwide, as its substitution should be demanded to a non-toxic reagent with similar efficacy as fixative.

ADDAX SCIENTIFIC RESULTS

Tissue fixation (a critical step in the diagnostic process) requires a reagent fit to preserve both, structure and cellular components (antigens/proteins and nucleic acid sequences). Substitution of Formalin (a toxic and carcinogenic agent) is recommended for environmental reasons and now required by EU rules Glyoxal (a di-aldehyde similar to Formaldehyde) is not volatile and is not toxic. When deprived of troublesome acids, Glyoxal Acid-Free (GAF) represents a safe histological fixative providing results as good or even better than Formalin, as proved by histological, immuno-histochemical and molecular analyses.

SCIENCE01

PRESERVATION OF CELL/TISSUE STRUCTURE AND ANTIGENS

Section of a colorectal adenocarcinoma fixed in GAF where preservation of structural and cell components can be appreciated *.

Tissue fixation (a critical step in the diagnostic process) requires a reagent fit to preserve both, structure and cellular components (antigens/proteins and nucleic acid sequences). Substitution of Formalin (a toxic and carcinogenic agent) is recommended for environmental reasons and now required by EU rules Glyoxal (a di-aldehyde similar to Formaldehyde) is not volatile and is not toxic. When deprived of troublesome acids, Glyoxal Acid-Free (GAF) represents a safe histological fixative providing results as good or even better than Formalin, as proved by histological, immuno-histochemical and molecular analyses.

SCIENCE02

PRESERVATION OF CELL/TISSUE STRUCTURE AND ANTIGENS

HER2 overexpression immuno-histochemically assessed in a GAF-fixed gastric adenocarcinoma*

PRESERVATION OF NUCLEIC ACIDS FOR MOLECULAR ANALYSES

Molecular DNA analysis of a GAF-fixed sample of carcinoma of the colon (Direct sequencing, Pyrosequencing, Mass Spectrometry, from left to right)*

SCIENCE04

PRESERVATION OF NUCLEIC ACIDS FOR MOLECULAR ANALYSES

Molecular DNA analysis of a GAF-fixed sample of carcinoma of the colon (Direct sequencing, Pyrosequencing, Mass Spectrometry, from left to right)*

*Reference: Bussolati G, Annaratone L, Berrino E, Miglio U, Panero M, Cupo M, Gugliotta P, Venesio T, Sapino A, Marchiò C.
Acid-free glyoxal as a substitute of formalin for structural and molecular preservation in tissue samples. PLoS One. 2017 Aug 10;12(8):e0182965.

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