GAF VALIDATION

Tissue fixation, leading to preservation of structural components, plays a critical role in histopathological diagnosis. The fixative of choice is still represented by Formalin (a water solution of Formaldehyde). This reagent is toxic and cancerogenic, but is still used worldwide in lack of alternative fixatives guaranteeing similar results. GAF (Glyoxal, acid free)  Fixative is an innovative reagent that allows optimal tissue fixation at structural and molecular level combined with the absence of toxicity and carcinogenic activity. The activity of research and technological transfer has been performed at the University of Torino and the results have been published in an international peer reviewed journal (Plos One 12:e0182965; 2017).
Validation of GAF fixative, as compared to Formalin was conducted on parallel samples, obtained with core needles or punch devices from surgical specimens and fixed alternatively in Formalin and in GAF. The first Validation trial was conducted in December 2017 at the San Giovanni Battista Hospital of Turin, Italy. Slides from 10 pathological cases (mainly of Colon Cancers) were presented, blindly to  pathologists of the University of Torino. The results confirmed the feasibility and validity of GAF fixation as alternative to Formalin fixation.

The slides (10 cases in parallel, fixed either in Formalin or in GAF) of this first Trial are now presented, for the open demonstration of the results obtained.

MAIN RESULTS

n. 24230/17 Formalin Fixation – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14351/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=1.5625&x=92192&y=42240

• n. 24230/17 GAF – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14354/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=69136&y=49280

• n. 24748/17 Formalin Fixation – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14366/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=63376&y=28160

• n. 24748/17 GAF – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14363/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=1.5625&x=88352&y=45056

• n. 24837/17 Formalin Fixation – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14357/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=57616&y=32384

• n. 24837/17 GAF – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14360/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=26896&y=18304

• n. 25462/17 Formalin Fixation -A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14369/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=76816&y=38016

• n. 25462/17 GAF – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14375/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=55696&y=40832

• n. 25921/17 Formalin Fixation – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14372/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=0.78125&x=23104&y=45056

• n. 25921/17 GAF – B URL
https://ome-cytest.crs4.it:9090/webclient/img_detail/14378/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=30736&y=52096

• n. 25936/17 Formalin Fixation – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14384/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=21136&y=23936

• n. 25936/17 GAF – A URL
https://ome-cytest.crs4.it:9090/webclient/img_detail/14381/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=1.5625&x=42272&y=36608

• n. 26021/17 Formalin Fixation – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14390/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=0.9566134377824645&x=13328.267716535434&y=40141.6062992126

• n. 26021/17 GAF – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14387/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=38416&y=52096

• n. 26315/17 Formalin Fixation – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14393/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=3.125&x=97936&y=40832

• n. 26315/17 GAF – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14396/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=0.78125&x=84544&y=33792

• n. 26422/17 Formalin Fixation – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14402/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=1.9975896354323592&x=16595&y=19223.16742081448

• n. 26422/17 GAF – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14399/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=2.1998945456675845&x=17069&y=17455.382157123833

• n. 27004/17 Formalin Fixation – B
https://ome-cytest.crs4.it:9090/webclient/img_detail/14408/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=2.056329364207566&x=11403.81517094017&y=18674.05128205128

• n. 27004/17 GAF – A
https://ome-cytest.crs4.it:9090/webclient/img_detail/14405/?c=1%7C0:255$FF0000,2%7C0:255$00FF00,3%7C0:255$0000FF&m=c&p=normal&ia=0&q=0.9&t=1&z=1&zm=2.180888061208463&x=22307.426440329218&y=17607.506172839505

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